2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) CAS# 30931-67-0
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) , Promotion Season Now in Store and Free Sample for Testing with Factory Price
- Appearance: Yellowish green to green powder
- Assay: 98.0%min
- Packaging: 500g fluorinated bottle.
- Sample: Available
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate): The Complete Guide
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Quality Control of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
Quality Control & MSDS
Packing of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
Description of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
In biochemistry, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) or ABTS is chemical compound used to observe the reaction kinetics of specific enzymes. A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect for binding of molecules to each other.
It is commonly used as a substrate with hydrogen peroxide for a peroxidase enzyme (such as horseradish peroxidase) or alone with blue multicopper oxidase enzymes (such as laccase or bilirubin oxidase). Its use allows the reaction kinetics of peroxidases themselves to be followed. In this way it also can be used to indirectly follow the reaction kinetics of any hydrogen peroxide-producing enzyme, or to simply quantify the amount of hydrogen peroxide in a sample.
The formal reduction potentials for ABTS are high enough for it to act as an electron donor for the reduction of oxo species such as molecular oxygen and hydrogen peroxide, particularly at the less-extreme pH values encountered in biological catalysis. Under these conditions, the sulfonate groups are fully deprotonated and the mediator exists as a dianion..
This compound is chosen because the enzyme facilitates the reaction with hydrogen peroxide, turning it into a green and soluble end-product. Its new absorbance maximum of 420nm light (ε = 3.6 × 104 M–1 cm–1) can easily be followed with a spectrophotometer, a common laboratory instrument. It is sometimes used as part of a glucose estimating reagent when finding glucose concentrations of solutions such as blood serum.
ABTS is also frequently used by the food industry and agricultural researchers to measure the antioxidant capacities of foods. In this assay, ABTS is converted to its radical cation by addition of sodium persulfate. This radical cation is blue in color and absorbs light at 734nm. The ABTS radical cation is reactive towards most antioxidants including phenolics, thiols and Vitamin C. During this reaction, the blue ABTS radical cation is converted back to its colorless neutral form. The reaction may be monitored spectrophotometrically. This assay is often referred to as the Trolox equivalent antioxidant capacity (TEAC) assay. The reactivity of the various antioxidants tested are compared to that of Trolox, which is a water-soluble analog of vitamin E.
Basic Info of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
2,2′-Azinobis(3-ethylbenzothiazolin-6-sulfonsaeure)diammoniumsalz;AzBTS-(NH4)2;2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt;ABTS (diammonium salt);ABTS SUBSTRATE;Expand
Appearance & Physical State
Light green powder
881.2ºC at 760mmHg
Store at RT.
What is 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)?
With the progress of science and technology and the development of society, chemical products have invariably permeated our daily lives, in medicine, food, cosmetics, electronics, industry, and other areas, becoming an essential part of our lives. One such product is Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) which has developed particularly rapidly in recent years. Do you know about Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) ?
2,2′-Bianisobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is a chemical compound with the chemical formula C18H24N6O6S4 and a molecular weight of 548.68.
What’s the application of Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) ?
A peroxidase substrate suitable for ELISA steps that produce a soluble green end product that can be observed spectrophotometrically at 405 nm; spectroscopic reagent for free chlorine, enzyme-free colorimetric substrate, peroxidase substrate
Free chlorine spectroscopy reagent, enzyme-free chromogenic substrate, peroxidase substrate, for ELISA steps, which produces a soluble greenish end product that can be read spectroscopically at 405 nm. This reaction can be terminated with 1% SDS.
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