Guanidine thiocyanate CAS# 593-84-0
Guanidine thiocyanate, Promotion Season Now in Store and Free Sample for Testing with Factory Price
- Appearance: White crystal powder.
- Assay: 99.5%min.
- Packaging: 25kg/fiber drum.
- Sample: Available.
Guanidine thiocyanate: The Complete Guide
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Quality Control of Guanidine thiocyanate
Quality Control & MSDS
Packing of Guanidine thiocyanate
Description of Guanidine thiocyanate
Guanidinium thiocyanate (GITC) is a chemical compound used as a general protein denaturant, being a chaotropic agent, although it is most commonly used in the extraction of DNA and RNA.
Note: this compound may also be recognized as guanidine thiocyanate. This is because guanidinium is the conjugate acid of guanidine and is called the guanidinium cation, [CH6N3]+.
Guanidinium thiocyanate can be used to deactivate a virus, such as the influenza virus that caused the 1918 “Spanish flu”, so that it can be studied safely. This application is presumably based on its denaturation activity.
Guanidinium thiocyanate is also used to lyse cells and virus particles in RNA and DNA extractions, where its function, in addition to its lysing action, is to prevent activity of RNase enzymes and DNase enzymes by denaturing them. These enzymes would otherwise damage the extract.
A commonly used method is guanidinium thiocyanate-phenol-chloroform extraction. It is not strictly necessary to use phenol or chloroform if extracting RNA for Northern blotting or DNA for Southern blot analysis because the gel electrophoresis followed by transfer to a membrane will separate the RNA/DNA from the proteins. Additionally, since these methods use probes to bind to their conjugates, peptides that get through the process don’t generally matter unless a peptide is an RNase or DNase, and then only if the enzyme manages to renature, which should not occur if proper protocols are followed. A possible exception might be when working with temperature extremophiles because some enzymes of these organisms can remain stable under extraordinary circumstances.
Basic Info of Guanidine thiocyanate
R20/21/22; R32; R52/53
P260; P280; P301 + P312 + P330; P303 + P361 + P353; P304 + P340 + P310; P305 + P351 + P338
Supplemental Hazard Statements
Contact with acids liberates very toxic gas.
H302 + H312 + H332; H314; H412
Appearance & Physical State
1.103 g/mL at 20ºC
132.9ºC at 760 mmHg
Stability Stable, but light sensitive. Incompatible with acids (contact releases very toxic gas), strong oxidising agents.
Store at RT.
What is Guanidine thiocyanate?
With the progress of science and technology and the development of society, chemical products have invariably permeated our daily lives, in medicine, food, cosmetics, electronics, industry, and other areas, becoming an essential part of our lives. One such product is Guanidine thiocyanate which has developed particularly rapidly in recent years. Do you know about Guanidine thiocyanate?
The official answer: Guanidine thiocyanate is an organic compound with the molecular formula C2H6N4S.
What’s the application of Guanidine thiocyanate?
Guanidine thiocyanate can be used as a biochemical reagent, pharmaceutical intermediate. It is used for degreasing in the oil and grease industry, as a strong lipid degradation agent in the daily chemical industry, and cell membrane rupture agent in biopharmaceuticals. The principle of RNA extraction by guanidine isothiocyanate-phenol method: guanidine isothiocyanate and b-mercaptoethanol work together to inhibit the activity of RNase, while guanidine isothiocyanate and sodium dodecyl sarcosinate Chemicalbook work together to denature the protein, thus releasing RNA; under acidic conditions DNA rarely dissociates and is precipitated along with protein denaturation, while RNA is dissolved in the supernatant in the supernatant. Guanidine thiocyanate has been used:- to lyse colorectal adenocarcinoma cell lines before RNA isolation- as a PCR inhibitor for triple mitochondrial DNA (mtDNA) qPCR assaysGuanidine thiocyanate is a denaturant and is often Chemicalbook used for RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol-chloroform in RNA extraction. Chemical depilatories are depilatories that soften the hair, reduce the strength of the hair, and wipe or rinse off the hair within 2-6 min. Because the composition of the hair stem is similar to the skin, chemical depilatories will inevitably damage the skin, so the depilatory agent is classified as a special purpose cosmetic. Thioglycolic acid type depilatory agent is the main depilatory agent nowadays, and it gradually replaces sulfide depilatory agent. These hair removal agents use hair removal active substances for thioglycolic acid and its salt, thioglycolic acid and its salt, thioglycolic Chemicalbook lactic acid, and it’s salt. From the performance and price considerations, the main use of thioglycolic acid and its salts for hair removal active agent. The application of some swelling agents (Swellingagents) helps to accelerate the speed of the hair removal action of mercapto compounds, thus, reducing the irritating effect on the skin. Such swelling agents include melamine, dicyandiamide, or a mixture of both (w = 0.5% to 2%), thiourea, potassium thiocyanate, guanidine thiocyanate, and PVP copolymer. They are mainly used in biopharmaceuticals, chemical reagents, etc. Inactivating agent (Chaotropic agent) and strong denaturing agent for denaturing lysis of cells; extraction of RNA and DNA.
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